Dados do Trabalho

Título

CEREBRAL OXIDATIVE STRESS IN SYSTEMIC INFECTION AFTER ISCHEMIC STROKE

Resumo

Larissa Joaquim1, Beatriz Steiner1, Lucineia Gainski Danielski2, Fernanda Gava2, Richard Simon Machado1, Brenno Farias1, Khiany Mathias1, Jaqueline da Silva Generoso2, Rafael Mariano de Bitencourt1, Fabricia Petronilho1

1 University of Souther Santa Catarina, Tubarão, SC, Brasil.

2 Laboratory of Cerebrovasculares Diseases (DCeV), Postgraduate Program in Health Sciences, University from Extreme South Catarinense, Criciúma, SC, Brasil.

ABSTRACT:

Introduction: Ischemic Stroke (AVCi) represents 87% of all stroke, and in Brazil already reached 222.773 thousand individuals. AVCi has a greater effect in women than in men, then women have more events and are less likely to recover. The pathophusiological mechanisms of AVCi determine evenst suc as neuroinflammation and oxidative stress, leading the individual to a systemic imune suppression, being sepsis one of them. The role of oxidative stress associated with inflammatory conditions is well characterized, event that can be observed in brain áreas such as the prefrontal cortex and hippocampus. Objective: To evaluate the relationship between AVCi and sepsis through oxidative parameters in female rats submetted to the animal model of AVCi and sepsis. Methodology: Female Wistar rats aged 60 days were randomized into groups: SHAM+SHAM, SHAM+MCAO, SHAM+CLP, MCAO+CLP, and submitted on day 0 to Middle Cerebral Artery Occlusion (MCAO) and on day 7 submitted to Cecal Ligation and Perforation (CLP) technique, suffering painless death after 24 hours and the hippocampus and prefrontal cortex were removed for biochemical analysis. Result: We observed that sepsis exacerbated reactive oxygen and nitrogen species in animals that suffered AVCi as well as increased sensitivity of lipid peroxidation in sepsis. The brain damage caused by AVCi induces protein oxidation and the association of two conditions was effective to potentiate a decrease of antioxidant enzyme catalase decrease. Conclusion: Sepsis can potentiate brain oxidative stress in female rats undergoing AVCi.

Palavras Chave

ischemic stroke, sepsis, oxidative stress, neuroinflammation, brain.