Dados do Trabalho

Título

ANALYSIS OF PRO1170ALA POLYMORPHISM OF THE HER-2 GENE IN BREAST CANCER PATIENTS: CORRELATION BETWEEN GENETIC EVALUATION AND CLINICAL ASPECTS.

Introdução

The overexpression of receptor 2 of the human epidermal growth factor (HER-2) is related to differentiation of malignant tumor cells, which predisposes worse prognoses in individuals with breast cancer. There are still not enough studies to clarify the relationship of single nucleotide polymorphisms (SNP) of the ErbB-2 gene, such as Pro1170Ala, to breast cancer and the clinical outcome, although it consists of one of the most common changes of this gene. Given this, it is essential to study this polymorphism to determine a better understanding of the molecular characteristics of cancer and analyze the impacts of this genetic variation on the prognosis and course of the disease.

Objetivo

Correlate Pro1170Ala polymorphism with the clinical aspects of women with breast cancer and assess its implications on the prognosis and evolution of this disease.

Método

Observational, descriptive and cross-sectional study with 171 healthy women and diagnosed with breast cancer between January 2014 and December 2021 in Londrina - PR. Clinical data obtained retrospectively in medical records and in questionnaires applied to the population were analyzed. Samples of biological material were collected by oral swab with sterile microbrush brush. The extracted DNA was amplified by polymerase chain reaction (PCR) technique with specific primers and genotyped by electrophoresis. The statistical analysis of pro1170Ala polymorphism occurred within the Hardy-Weinberg equilibrium. Odds Ratio was calculated with a 95% confidence interval using Fisher's exact test. Kendall's Tau-b correlation coefficient was used to determine the link between the SNP studied and the studied SME. The results obtained were calculated by the Software SPSS Statistics 22.0.

Resultado

The study did not demonstrate an association between Pro1170Ala polymorphism and susceptibility to breast cancer and clinical aspects and prognosis. There was a positive significance between the studied polymorphism and HER-2 overexpression (p= 0.009). Bivariate analysis of clinical data showed a correlation between body mass index and arterial hypertension (p= 0.0001) and tumor size (p= 0.037). Furthermore, associations were identified between the presence of estrogen and progesterone hormone receptors (p=0.0001) and tumor size with arterial hypertension (p= 0.037).

Conclusão

Positive results between hypertension and tumor size and the expression of both hormone receptors are findings that need to be confirmed in studies with a larger number of samples.