Dados do Trabalho

Título

Establishment of paired primary cell cultures from osteosarcoma patients as new tool for better understanding of the tumorigenesis, resistance and metastasis

Resumo

Osteosarcoma is the most common malignant bone tumor in children and adolescents. The 5-year survival rate in patients with localized OS reaches 70%, but for metastatic disease, the survival rate drops to only 20%. Primary cell cultures obtained from different sites could be useful to better understanding the osteosarcoma. Thus, the aim of the present study was to establish paired primary cultures from osteosarcoma patients. Therefore, 66 specimens obtained from 39 patients were processed. Among them, 51 specimens were obtained from 26 patients diagnosed with osteosarcoma. All cryopreserved primary cultures were established from 15 primary tumors pre-chemotherapy, 12 primary tumors post-chemotherapy, 5 lung metastases, 5 bone metastases, 1 lymph node metastasis, 1 local relapse, 1 normal bone surrounding the tumor and 1 non-malign bone. Five primary cultures were maintained in culture until the 30th passage, three were investigated regarding the osteosarcoma marker, SATB2, and one was investigated regarding vimentin and p53, being positive to all markers. In summary, this is the first study that established paired primary cell cultures obtained from osteosarcoma patients, regarding chemotherapy exposition, primary or metastatic site, tumor or normal bone surrounding the tumor, and relapse. Due to the known inter-patient heterogeneity in osteosarcoma, further investigations using the paired cultures could define important points, still unclear, regarding resistance and metastasis. Therefore, these primary cell cultures could be useful to better understanding of the tumorigenesis, treatment response and progression in osteosarcoma.