Dados do Trabalho

Título

CIRCADIAN RESPONSE OF PRO AND ANTI-INFLAMMATORY CYTOKINES IN CHILDREN AND ADOLESCENTS WITH OSTEOSARCOMA: A CROSS-SECTIONAL PILOT STUDY

Introdução

Introduction: Circadian rhythm is pivotal in several physiological processes, including sleep/awakening and cancer progression. In osteosarcoma, a deep understanding of the complex cytokine interplay in oncogenesis may have prognostic significance for its course/progression.

Objetivo

Objective: We aimed to measure cytokines levels in saliva of pediatric patients with osteosarcoma undergoing chemotherapy for osteosarcoma in 4 periods a day (1/4/9/14h after awakening), in order to characterize the cytokines circadian rhythm in a day of hospitalization and compare the findings between metastatic and non-metastatic patients.

Método

Methods: A cross-sectional pilot study was undertaken in a Brazilian Hospital. Six children and adolescents with a diagnosis of osteosarcoma receiving the same chemotherapy protocol were included and have donated 4 saliva samples (2 ml) via passive drool technique on a day of hospitalization. ELISA technique was used to evaluate the cytokines levels.

Resultado

Results: Most participants were male (66%), average age=12.5, and 33% had pulmonary metastasis. At the +1h awakening the average of TGF-β concentration in non-metastatic patients was higher (210pg/ml) than average of TGF-β in metastatic patients (40pg/ml). At the +14h awakening the average of IL-6 concentration and TNF-α in metastatic patients showed respectively an increase levels (53pg/ml;120pg/ml) compared to the non-metastatic patients (35pg/ml;97pg/ml). The average in all 4 collection points of IL-1β concentration was significant higher in metastatic patients than in non-metastatic patients (p=0.01).

Conclusão

Conclusion: These results pointed out to preliminary evidence on the circadian response of pro and anti-inflammatory cytokines in children and adolescents with osteosarcoma. Furthermore, there are still several gaps in the literature to be addressed regarding the circadian rhythm in an attempt to improve osteosarcoma immunotherapy, given the clinical heterogeneity and the variable and individual immune response of the patients.

Palavras-chave

Keywords: Circadian rhythm; Cytokines; Pediatric Oncology; Osteosarcoma.

Área

Oncologia - Tumores cutâneos e Sarcomas